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For comparison between two groups, Students t -test was performed

During the analysis, the Variable Importance in Projection (VIP) is used to quantify the contribution of each metabolite and its discriminative value for sample classification, which is of significant guidance for the screening of potential biomarkers

NAD + and NADH measurements (enzymatic assay) The frozen liver tissue (approximately 50 mg) was lysed in 500 l of ice-cold 0.6 M perchloric acid with a metal bead in the TissueLyser (Qiagen), with a frequency of 20 Hz for 2 min

The beneficial effects of GLP-1 receptor activation on glycemia occur through several mechanisms: GLP-1 stimulates glucose-dependent insulin secretion from pancreatic -cells, suppresses inappropriately elevated glucagon secretion from pancreatic -cells, delays gastric emptying, and induces satiety via a direct action in the central nervous system