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The novel object recognition test was performed on day p91

chalepensis aerial part aqueous extract at doses of 100, 300, and 600 mg/kg in rats revealed no deaths or significant changes in body weight

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Because of the retrospective nature of the study and the use of de-identified data, this study received an exemption waiver for approval and informed consent from the Institutional Review Board (IRB) of Chung Shan Medical University Hospital, identified by the reference number CS1-24047

Given that an average rat weighing 200 g drinks approximately 4050 ml/day [120], it would consume 3645 mg of NAC daily, corresponding to 180225 mg/kg/day