IV delivery bypasses digestion entirely, placing glutathione directly into the bloodstream at near-100 percent bioavailability
In addition, GSH levels may also be determined by nutritional status, some hormones, psychogenic stress, and physical activity and may also exhibit diurnal variations [8]
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Cell lines and culture conditions HepG2 cells, a human hepatoma cell line, were obtained from the American Type Culture Collection (Manassas, VA) and were cultured in Dulbeccos modified Eagles medium (DMEM) containing 10% (v/v) fetal bovine serum, 2 mM glutamine, 5 U/mL penicillin, and 50 g/mL streptomycin at 37 C in a humidified O 2 /CO 2 (19:1) atmosphere
It has been approved for clinical use in the therapy of angina pectoris [210, 211]