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Brain sections at bregma1 mm ( n = 4 per group) were deparaffinized and treated with xylene and decreasing graded ethanol to distilled water for hydration

To minimize discomfort, ensure the pen is at room temperature before injection, and apply a cold compress after the dose is administered

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In preclinical settings it may be used to explore endocrine axis dynamics by tracking circulating biomarker changes in animal models, alongside tissue level gene expression panels and proteomic readouts