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10.1016/j.bbalip.2007.02.012 Biochim

IGFBP-3, insulin-like growth factor-binding protein 3

In addition, diverse statistical methods were applied to examine the utility of the three parameters as multidimensional biomarkers for predicting ASD risk prior to the onset of behavioral abnormalities or for predicting the developmental trajectory of children with ASD

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Immediately after cells were washed twice with 1 mM MgCl 2 in PBS (pH 6.0) and incubated with a staining solution composed of 1 mg ml 1 5-Bromo-4-chloro-3-indolyl--D-galactopyranoside (prepared in N , N -dimethylformamide), 0.12 mM K 3 Fe(CN) 6 , 0.12 mM K 4 Fe(CN) 6 and 1 mM MgCl 2 /PBS (pH 6.0) at 37 C overnight in CO 2 -free incubator