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Participants height, body mass, hematocrit and sex were used to calculate blood, plasma and RBC volume using the Nadler equation as previously published ( 3.2 RBC indices, plasma haptoglobin concentration, lactate levels and RBC electrolyte concentration RBC count, hemoglobin concentration (hb) and hematocrit (hct) were unaffected by the race

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The qPCR cycling conditions were as follows: an initial denaturation at 95 C for 15 min, followed by 30 cycles of 95 C for 7 s and 58 C for 10 s, and a final extension step at 95 C for 5 min, then 40 cycles of 95 C for 15 s and 58 C for 30 s