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50 L of the cell suspension was mixed with precooled 240 L 50% PEG 3350, 10 L of plasmid pRS315-scCHC_mScarlet carrying CHC1 gene tagged with mScarlet and LEU selection marker and 25 L of 2 mg/mL ssDNA was added and vortexed

4.3 Reproduction To ensure a required gene expression pattern to support embryonic development, the maternal (oocyte) and paternal (sperm) epigenome are remodeled to reach totipotency, driving cellular differentiation toward all cell types (Ross and Sampaio, 2018), including embryonic and extraembryonic ( i.e ., placenta) cell types

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