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For in vitro cytotoxicity assays where benzyl alcohol vehicle effects could confound results, include matched Bacteriostatic Water vehicle controls at the same final benzyl alcohol concentration as the peptide treatment to distinguish compound-specific from vehicle-associated biological effects

10.1038/s41419-021-04367-3 97

GlyNAC is a 1:1 combination of glycine and N-acetylcysteine

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After coincubating freshly isolated and activated mouse T cells with particles encapsulated with EGFP or luciferase mRNA for 24 h, the CD5/ LNP group exhibited significantly higher EGFP or luciferase expression rates than did the unmodified LNP group (Fig