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DMEM, Dulbeccos Modified Eagle Medium

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MCF-7 Cas9-expressing cells were transduced in independent triplicate samples of 1.73 10 8 cells each with the lentiviral whole-genome sgRNA library at a multiplicity of infection of 0.25-0.29, as measured by puromycin resistance, to achieve a representation of 252287 cells per sgRNA

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Immunity 56 , 5877.e11 (2022)

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