Next, 100 L samples of the 10 5 , 10 6 , and 10 7 dilutions were transferred onto 10 different fungal culture media: potato dextrose agar (PDA), Sabouraud dextrose agar (SDA) (Akgl and erikiolu, 2009), malt extract agar (MEA) (Chapman et al., 2007), modified Leeming and Notman agar (MLNA) (Hsieh et al., 2018), carrot agar medium (CAM), modified Melin-Norkrans medium (MMN) (Vuorinen et al., 2015), fungal isolation medium (FIM) (Arifin et al., 2021), modified Dixon agar (mDixon) (Bond and Lloyd, 1996), panfungal medium (PF), and corn meal agar (CMA) (Walker and Huppert, 1960)
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Nucleotide bases were incorporated in different numbers between the primer and adapter sequence to increase the overall sequence diversity of the samples, thereby improving the quality of the sequenced data
