The supernatant was passed through a mouse anti-human AIM IgG monoclonal antibody (CL7)-immobilized HiTrap NHS-activated HP column and the protein was eluted with 0.1 M glycine-HCl (pH 2.5) and neutralized with 1 M Tris-HCl (pH 8.5)
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Abbreviations ATF3: Activating transcription factor 3 BiP: Endoplasmic reticulum chaperone BiP ER: Endoplasmic reticulum GLP-1: Glucagon-like peptide 1 GLP-1R: Glucagon-like peptide 1 receptor HPF: Hydroxyphenyl fluorescein iPSC: Induced pluripotent stem cell JunB: Transcription factor JunB KIRRE2: Kin of IRRE-like protein 2 KO: Knockout NPC: Neural precursor cell OCR: Oxygen consumption rate OCT4: Octamer binding protein 4 siCT: Control siRNA WFS1: Wolframin WT: Wild-type ZIC1: Zinc finger protein ZIC 1 References Fraser FC, Gunn T (1977) Diabetes mellitus, diabetes insipidus, and optic atrophy
24 These patients should be offered one of these medications in the absence of contraindications, regardless of glucose control