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The resulting amplicon was digested with Nco I and Hin dIII and inserted into vector pET-28b, generating the pET-28b-MBP-TEV-R9 plasmid for expression of TEV with a His 6 -tag at its N-terminus and nine consecutive arginine residues at its C-terminus
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Averaging premium and discount volumes, FDA rules may affect about 4% of the total cigarette volume
The first group included patients who smoked both cannabis and tobacco, defined as smoking at least 10 joints per month for at least 1 year and at least 1 pack-year of tobacco
8 Marijuana users are exposed to a greater concentration of carcinogens than tobacco users
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