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Growth of methionine synthesis mutants in laboratory cultures The L-methionine synthesis mutants were first grown in 7H9-Tween-ADC liquid medium, both with and without B12 (AdoCbl, 10 g/mL), to assess their growth in the presence and absence of the vitamin and thus validate their phenotypes

The combination of pipetting and forcing sample volume through the micro-column in place at the distal end of a pipette tip provides enhancement of the enrichment process due to microfluidics, resulting in shorter incubation times

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The long-term effects on humans remain unknown

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