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PCR was performed in a final volume of 50 l containing 2 l of the single strand cDNA product (10 ng/l), 10 mM Tris-HCl (pH8.3), 50 mM KCl, 1.5 mM MgCl 2 , 200 M dNTPs, 1.25 U Taq (Takara), and 20 pmol of each forward (5) and reverse (3) primers
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