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The PCR conditions consisted of denaturation at 95 C for 3 min, followed by 40 cycles of denaturation at 95 C for 30 s, annealing at 58 C for 30 s and extension at 72 C for 30 s

The tricarboxylic acid cycle, an ancient metabolic network with a novel twist

Case Presentation A 71-year-old female with no prior psychiatric history presented with sudden onset of: Severe anxiety Insomnia Cognitive dysfunction Fatigue Severe neck and shoulder pain Abdominal pain New intolerance to previously well-tolerated foods Symptoms began within months of moving into a new office building , suggesting a potential environmental trigger

doi:10.1002/14651858.CD001044

MHB (160 l) was added to the first column of a 96-well plate and 90 l MHB to each of the remaining columns