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Taxonomic annotation of OTUs were carried out using SILVA database

This can lead to emphysema, a form of COPD (chronic obstructive pulmonary disease)

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107 In the US, state legislation restricting sale of tobacco to minors often includes legislative restrictions for possession, use or purchase of tobacco by minors, however these laws (sometimes referred to as PUP laws) appear to have little impact on reducing smoking prevalence

The authors further note that HTP aerosol temperatures can still reach levels sufficient for nicotine pyrolysis, potentially eliciting similar oxidative responses to those from combustible cigarettes