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The chromatographic procedures involved the use of a C 18 column (100 2.1 mm, 1.7 m) with a binary mobile phase mixture of methanol and water (50:50, v/v) at a flow rate of 0.3 ml/minute in a total run time of 3.8 min

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These improvements also allowed for the release of ionized nicotine, which could be absorbed by the lower parts of the lung (including the tracheal and large bronchi)

57 and n=19 in both arms of Polosa et al