Transcription of apoptosis-related genes by quantitative real-time PCR This work utilized a quantitative real-time PCR approach with SYBR green detection to evaluate MALAT1, LUCAT1, BANCR, P53, BAX, BCL2, and AKT3 transcription levels
Schssler, P., Yassouridis, A., Uhr, M., Kluge, M., Weikel, J., Holsboer, F., & Steiger, A
In recent years, oxidative stress has been postulated to be an important factor in the pathogenesis and development of lifestylerelated disease, such as gastroesophageal reflux.21 It is strongly agreed that ROS and reactive nitrogen species (RNS) are generated during inflammation and are considered to contribute to flogosis leading to carcinogenesis.40 In fact, chronic inflammation during GERD is an important risk factor of Barrett's oesophagus (BE) and oesophageal carcinogenesis.3 The goal of reflux treatment is not necessarily, the complete absence of symptoms, the healing of major oesophageal lesion and the prevention of complications.41 ROS and RNS can induce the formation of a variety of molecule markers of oxidative and nitrosative damage, such as the production of superoxide anion (O 2 ) and nitric oxide (NO)
Similarly, the ET algorithm effectively captured complex interactions between variables, highlighting its suitability for clinical prediction tasks
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