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Our model provides a comprehensive assessment of disease progression through multiple validated endpoints: Clinical score Body temperature Body weight evolution Viral load (nasal washes, BAL, lungs and nasal turbinates) Histopathology The model consistently reproduces the key hallmarks of influenza infection, including clinical signs, transient fever, body weight loss, significant viral replication in respiratory tissues and characteristic inflammatory lesions

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The culture environment for all cells was 37 C, 5% CO 2

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Pratley and Gilbert 2008