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Acetonitrile (A) and 10 mM ammonium carbonate buffer, pH 9.3 (B) were used as the mobile phase, with gradient elution from 80% A to 20% A in 30 min, at a flow rate of 100 l/min

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Adapted from Ruiz-Colon et al, 2014), Recommended Treatment: No known antidote, or FDA-approved medications to manage xylazine overdose or withdrawal Xylazine is not detected in routine toxicology screens (Rubin, 2022) naloxone treatment does not appear to be of benefit (Ruiz-Colon et al, 2014) Maintain respiratory function & blood pressure & supportive care (Ruiz-Colon et al, 2014

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