tobacco mosaic virus electron microscopy Photograph by 'Centre For Bioimaging, Rothamsted Research / Science Photo Library' Tobacco mosaic virus, TEM -

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The virus suspension was purified by a low speed 4C centrifugation at 2,300 g for 5 min, followed by supernatant ultracentrifugation at 4C on a 20% sucrose (BDH AnalaR, England, UK) cushion for 2 h at 32,000 rpm in a SW 41 Beckman Coulter Optima L-80 XP rotor (Beckman Coulter, Harbor Boulevard, CA, USA)