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Lymphocytes Lymphocyte subpopulations were detected by flow cytometry using an acoustic focus cytometer (FACS Attune, Applied Biosystems, Foster City, CA, United States)

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Supplementation with 4,000 IU/day of vitamin D for 2 months significantly increased serum 25-(OH) D3 levels and markedly decreased the LH/FSH ratio, LH, and T concentrations, and improved pregnancy rates following ovulation induction (41)