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Extracts were clarified by centrifugation and filtration through a 0.45 m pore Spin-X filter (Corning), and the total protein concentration was determined by BCA assay

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Configuration: Copper (II) coordinates with nitrogen atoms from glycine and histidine, as well as the oxygen group from lysine

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Conversion of the concentration from mass% to mol/liter (moles of solute/liter of solution = molarity): For full table with Iron, Lithium, Magnesium, Potassium, Sodium and Strontium Chloride - rotate the screen