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At 80% flask confluency, cells were harvested using DPBS for washing and incubated with 1.5 ml of 0.25% trypsin EDTA/DPBS and poly-vinyl-pyrrolidone for 34 mins at 37C to allow detachment

Health Minister Dao Hong Lan said the ban was introduced due to the negative health impacts associated with e-cigarettes and heated tobacco products

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Only 3.2 percent of adults who had never smoked cigarettes tried them, but the CDC and some public health advocates have been alarmed by a dramatic rise in their use among teenagers