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Cells were cultured in Gibco 1X RPMI 1640 (Life Technologies) medium supplemented with 10% FBS, at 37C, 5% CO 2 , and 20% O 2 condition

Rather than running each compound separately, investigators studying joint stress, tendon overload, or wound-repair models can work from a single pre-verified formulation with documented purity across all four components

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KO of TBC1D9 , a TBC protein related to TBC1D9B, caused a mild accumulation of lysosomes in the cell periphery and a this phenotype was exacerbated by double loss of both TBC1D9 and TBC1D9B ( TBC1D9/TBC1D9B DKO) (Supplementary Fig