For carbonyl analysis, DNPH-coated glass fiber filters (ORBO 827, SUPELCO, CA, United States) followed by DNPH cartridges (Sep-Pak XPoSure Plus Short Cartridge, Waters, Milford, MA, United States) were used to assure collection of both particle- and gas-phase carbonyls ( Nicotine Analysis The GFF were extracted with 4 ml methanol
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Gas chromatographic determination of trans-3-hydroxycotinine, a major metabolite of nicotine in smokers
However, smoking cessation was associated with decreasing HCC risk in a dose-dependent manner, and light-to-moderate alcohol consumption was associated with decreased HCC risk