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Slides were washed three times in TBST before concurrent secondary application (1:500) in Block-B buffer with DAPI for 0 min and room temperature

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Adolescents who indicated I have only tried smoking once or I used to smoke sometimes, but I never smoke cigarettes now at baseline and then selected one of the following, I sometimes smoke cigarettes now, but I dont smoke as many as one a week, I usually smoke between one and six cigarettes a week, and I usually smoke more than six cigarettes a week at follow-up were classified as having progressed in their smoking