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DNase-treated RNA was reverse transcribed in 20 l of RT-PCR mix (50 mM Tris, pH 8.3, 3 mM MgCl 2 , 75 mM KCl, 50 mM dNTPs, 2.5 M oligo(dT) 20 , 5 mM DTT, 2 U RNaseOUT and 10 U SuperScriptIII (Invitrogen) at 50C for 1 h
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