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Avoid chugging large amounts at once, which may worsen nausea
The V3 to V4 region of the 16S rRNA gene was amplified via polymerase chain reaction (PCR) employing the 341 F and 805R primers, while the fungal internal transcribed spacer (ITS) region was amplified using the ITS-1F and ITS-1R primers
65 Puffing parameters also vary considerably between users, such as total puff volumes ranging from 84 to 732 ml for little cigars and 270 to 2089 ml for cigarillos, determined in one study
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