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For urine samples, a microwave digestion system (Multiwave GO, Anton-Paar, Graz, Austria) was used to digest 5 ml sample with 5 ml HNO 3 and 2 ml H 2 O 2 for 10 min at 100C

There are concerns that the COVID-19 pandemic has further increased alcohol use (161,162)

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In communities with a legal smoking age of 21, the 18 to 20 group was less likely to smoke than those communities where the smoking age is still 18