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Inhibition of SGLT2 reduces sodium reabsorption in the proximal tubule and enhances Na + delivery to the macula densa, promoting finally the TGF activation with consequent afferent arteriole vasoconstriction and reduction of the single-nephron-glomerular-filtration-rate ( Figure 1 )

The DAPI (4,6-Diamidine-2-phenylindole dihydrochloride, #C1002), N-acetyl-L-cysteine (#S0077) and the tubulin-tracker Green (#C1051S) was purchased from Beyotime Biotechnology (China)