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T cell activation Fresh whole blood samples collected in heparin were diluted tenfold with RPMI and 90 l of the diluted blood was incubated in 96-well flat-bottom tissue culture plates in a humidified incubator at 5% CO 2 , 37C for 2 days in the presence or absence of anti-CD3 + anti-CD28

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In some homes there are dozens of candles burning by the last night of Chanukah

Smoking was very en vogue in the 1960s, and many of the show's characters smoke throughout the series