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Total 1,500 bp sequences upstream from the translational start site of each gene were surveyed to predict cis -acting regulatory elements against the PlantCare database ( Plasmid Construction and Arabidopsis Transformation of RcGPX4 The CDS of RcGPX4 was amplified from the castor bean leaves by real-time (RT)-PCR and inserted into the pCambia1300 expression vector under the control of cauliflower mosaic virus 35S promoter (CaMV 35S) with Kpn I and Xba I linkers using the primers GGGGTACCATGCTTTGTAGTTCTTCAACTAGAT, and GCTCTAGATCACGCAACCCCCAGCAGTTTCTTA

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Liposomal and sublingual formulations attempt to bypass this problem by protecting glutathione from enzymatic degradation or delivering it directly across oral mucosa