Patent CN105154498a example 10 discloses Arg34GLP-1 (9-37) main peptide chain is coupled with side chain with carboxyl protected by tert-butyl (tBu), then coupled with Boc-His (Boc) -Aib-OH dipeptide in presence of HATU and TEA, reaction time is as long as 14 days, freeze-drying time is as long as 16 hours after HPLC purification by post-treatment, which is not good for industrial production, and Boc has no strong absorption of Trt in ultraviolet, which is not good for reaction monitoring, and Boc has no good stability of Trt, which results in generation of racemic impurities
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MUTATION OR GENETIC ENGINEERING C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE C12P21/00Preparation of peptides or proteins C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g
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