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(See here: Vitamin B12 Deficiency Test) Interruptions in the Methylation Cycle The methylation cycle can be interrupted at various points, either due to nutrient deficiencies or genetic mutations which affect the required enzymes

Freezing should be avoided at all stages because it may damage container integrity and affect product quality

sterile water is not presented as a preserved multi-access reagent

The protocol typically involves starting HCG when beginning testosterone therapy, though some practitioners add it later if testicular atrophy becomes noticeable