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However, the large molecular size of GLP-1 analogues presents significant formulation challenges for transdermal delivery

For analysis, samples were reconstituted in 35 L of a 1:1 mixture of LCMS-grade isopropanol and methanol, and subjected to liquid chromatography-mass spectrometry (LC-MS) as previously outlined, employing a high-resolution hybrid QExactive HF Orbitrap mass spectrometer (Thermo Fisher Scientific) set to data-dependent acquisition mode (Top 8) with the capability of switching between positive and negative ion polarities

Alpha lipoic acid (ALA) can reduce l-cystine into two l-cysteine molecules to facilitate a faster increase in reduced glutathione

In addition, IHC analysis revealed that the P-gp, LC3B, VDAC1, and Ki-67 levels were highest in the HGC-27R-EVs-shNC and HGC-27R-EVs-shNC/PTX groups with no significant difference, suggesting that the transmission of PTX resistance by EV-derived VDAC1 protein through autophagy led to a reduction in the sensitivity of GC cells to PTX ( Figures 6D and S3C )

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