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To better understand how changes in intracellular GSH metabolism might contribute to the expansion, survival, and function of CD8 + T cells upon chronic antigenic stimulation, we used an in vitro system (26) whereby activated transgenic or wild-type (WT) T cells were expanded in the presence of persistent antigenic stimulation with either specific antigen (OVA-I/gp100) or anti-CD3mediated stimulation and anti-CD28 costimulation for 7 days

Together, our findings reveal a direct link between melatonin signaling, immune cell metabolism, and type 2 airway inflammation, and identify the melatonin-NRF2-glutathione axis as a potential therapeutic target in asthma