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This pH falls squarely within the overlapping stability window of all three peptides: the copper tripeptide (5.0-7.0), the thymosin fragment (6.0-7.5, though stable from 4.5-7.5), and the body protection compound (6.5-8.5, though stable across wider range)

In the range of 5.010.0, a good linear relationship between R I447/I581 and pH was built with a regression equation of R I447/I581 = 11.950.56 pH ( R 2 = 0.998)

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Skin that tolerates daily application without irritation but shows clear benefits compared to baseline has found its appropriate concentration