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Extracted proteins were quantified using the Pierce BCA Protein Assay Kit (Thermo Scientific #23225)

The signal cascade is as follows: cAMP PKA CREB activation of transcription of genes dependent on the CRE element (cAMP Response Element), including proinsulin genes in pancreatic beta cells

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N Engl J Med 2001;345:851-60.ArticlePubMed 10

GHK-Cu (glycyl-L-histidyl-L-lysine copper complex) is a regenerative signaling peptide that binds and helps deliver bioavailable copper to tissues where repair and remodeling processes are active