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Conflicts of interest Frhbeck G received honoraria from Lilly, Novo Nordisk, Regeneron, AstraZeneca and Boehringer Ingelheim as a member of advisory boards, and honoraria for lectures as a member of the OPEN Spain Initiative

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Preservative solution is recommended, but if the product will be used relatively quickly and kept refrigerated, deionized water or a blend of both may be used.

Up-regulation of the PPP flux provides cells with R5P for nucleotide biosynthesis, as well as NADPH for biosynthesis and maintaining redox homeostasis (59, 67, 70)

Research protocols exist to eliminate guesswork