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These pieces were incubated in Ca/Mg-free Hanks balanced salt solution (HBSS, SigmaAldrich) containing 30 mM ethylenediaminetetraacetic acid (EDTA, SigmaAldrich) and 1.5 mM dithiothreitol (DTT, SigmaAldrich) at 37 C with shaking for three cycles of 10 min each

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