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Cells were maintained at 37 C in an incubator with 5% CO 2

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Indeed, treatment with relatively high doses of antioxidants can even be linked to potential adverse effects due to interference with reactive oxygen and nitrogen species involved in normal cellular signaling (Frein et al

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Neuroprotection: Preliminary studies indicate GHK-Cu may have neuroprotective properties, with potential relevance to neurodegenerative conditions through its anti-inflammatory and antioxidant mechanisms