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While continuous-flow peptide synthesis is being developed to address some of these limitations, it remains at an early stage of industrial implementation

Growth media was replaced with binding buffer [phenol-free DMEM containing 25 mM HEPES and 0.1% (w/v) BSA] containing 0.7 nM 125 I-Ex(939) or 0.15 nM 125 I-hGLP-1 and increasing concentrations of unlabelled ligand

(2023) Journal of Biological Chemistry Cell-based mechanistic studies comparing retatrutide, tirzepatide, and semaglutide effects on hepatic glucose production and thermogenic gene expression via glucagon signaling