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Then, TMB chromogen solution (CWBIO) was added, and the absorbance at 450 nm was measured via a SpectraMax plate reader (Molecular Devices)

For fluorescence staining, we incubated samples for 2 h with Alexa-488 and Alexa-594 conjugated donkey antibodies (1:200) against mouse and rabbit immunoglobulins (Supplementary Figs 6 and 7 are representative images from two sets of immunolabelled cultures of NPCs and neurons)

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