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GPX4 genetic silencing in vitro The lentiviral plasmids, either non-targeted vector (pLKO.1-shNT) or pLKO.1-shGPX4 plasmid DNAs (Sigma-Aldrich) were transfected along with packaging and envelop plasmids psPAX2 and pMD2.G into HEK293T cells

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Following propensity score matching (PSM), 140 cycles with L-carnitine pretreatment were successfully matched with 140 routine IVF cycles

Trial investigators emphasised that supportive care strategies including dietary adjustments, hydration, and use of standard medications were sufficient to manage these milder GI issues