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TCEP (the final concentration is 5 mM) and iodoacetamide (the final concentration is 10 mM) (Sigma, I1149-25G) for reduction and alkylation were added to the solution and incubated at room temperature for 30 min, respectively

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NTS were incubated in supplemented media M199 at 37 C in 5% CO 2 in air [37]

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Similarly, older men engaging in either resistance or endurance exercise showed post-session increases in serum IGF-1 and BDNF