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Characterising how specific amino acid changes in variant receptors alter binding affinity, Gs coupling efficiency, cAMP signalling, and MITF activation in response to MC1R agonist stimulation

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Anaerobic samples were stored in glass screw top vials and N 2 -purged samples were transferred to pressure-relief scintillation vials kept under N 2 atmosphere via 27-gauge insulin syringe

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