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Both endothelial cells and aortic sections were treated with 0.5% (v/v) Triton X-100 for 15 min, followed by three washes with PBS, each time for 5 min, and then blocked with 5% (v/v) bovine serum albumin (BSA, Sigma-Aldrich, B2064) at room temperature for 1 h

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Both IF regimens led to significantly greater reductions in fasting glucose and HbA1c compared to the control group, with no statistically significant difference observed between the two IF schedules (125)

The role of tryptophan metabolites in neuropsychiatric disorders