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As a result of these synthetic forms, immunoassays that are able to differentiate endogenous IGF-1 from the therapeutic forms have become highly sought after

RNA extraction and RTqPCR Total RNA from cells was isolated via TRIzol (Takara, Otsu, Japan) and subsequently purified with chloroform/isopropanol/ethanol, and cDNA was synthesized via the PrimeScript RT reagent Kit (Takara) according to the manufacturers instructions and used as the template for qRTPCR

Mass spectrometry The conversion of GSH into GSSG was monitored by mass spectrometry (Agilent 1290 UPLC/6540 Q-TOF)

doi: 10.1016/j.fct.2013.01.029 287 MileoAMMiccadeiS

IDA Agarose Beads The metal ions are fixed on cross-linked agarose beads by a chelating ligand, nitilotriacetic acid (NTA) or iminodiacetic acid (IDA)