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At the indicated time (20 min and 60 min) an aliquot of 70 L was removed from each experimental reaction and pipetting into 175 L of acetonitrile at 0 C to quench the reaction ( t = 0 min was determined using the reaction without plasma)

Wu Q, Xi DZ, Wang YH (2019) MicroRNA-599 regulates the development of Parkinsons disease through mediating LRRK2 expression